Mixotrophic Cultivation of a Native Cyanobacterium, Pseudanabaena mucicola GO0704, to Produce Phycobiliprotein and Biodiesel

• Published in: Journal of microbiology and biotechnology Vol. 32; no. 10; pp. 1325 - 1334
• Main Authors: Kim, Shin Myung, Bae, Eun Hee, Kim, Jee Young, Kang, Jae-Shin, Choi, Yoon-E
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.10.2022
• Subjects: Biofuels - analysis; Biomass; Cyanobacteria - genetics; Fatty Acids - metabolism; Microalgae - metabolism; Phycobiliproteins - metabolism; Research article; Sodium Acetate - metabolism; organic carbon sources; Biofuel; biodiesel quality; cyanobacteria; phycobiliprotein
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2207.07008

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as GO0704 through 16s RNA analysis. The potential exploitation of GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].