Evaluation of Primers OPF-01, P54, and 1253 to Identify A. fumigatus , A. flavus , and A. niger from Polymorphic Patterns Obtained by RAPD-PCR

• Published in: Pathogens (Basel) Vol. 13; no. 7; p. 574
• Main Authors: Castro-Fuentes, Carlos Alberto, Frías-De-León, María Guadalupe, González-Villaseñor, María Del Carmen, Duarte-Escalante, Esperanza, Valencia-Ledezma, Omar Esteban, Martínez-Gamboa, Areli, Meraz-Ríos, Beatriz, Reyes-Montes, María Del Rocío
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.07.2024
• Subjects: A. flavus; A. fumigatus; A. niger; Aspergillosis; Clustering; DNA polymerase; evaluation; Gene sequencing; Identification; Immunocompetence; Maximum likelihood method; Phylogenetics; Phylogeny; Primers; Random amplified polymorphic DNA; RAPD-PCR; Regression analysis; Regression models; Species; Strains (organisms); United States > US; Mexico; evaluation; RAPD-PCR; A. niger; primers; A. fumigatus; A. flavus
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens13070574

We evaluated the specificity of the primers OPF-01, P54, and 1253 to identify , , and , respectively, with the RAPD-PCR method. Eighty-two isolates belonging to the sections , , and were used. The isolates were identified by phenotypic (macro- and micromorphology) and genotypic (partial sequences of the gene) methods. The RAPD-PCR method was used to obtain polymorphic patterns with the primers OPF-01, P54, and 1253. The specificity of the polymorphic patterns of the isolates of each species was evaluated through the UPGMA clustering method and logistic regression model. All isolates of the genus were identified at the section level by macro- and micromorphology showing the typical morphology of the sections , , and , and the species were identified by the construction of the phylogeny of the partial sequence of the gene. The patterns' polymorphic strains obtained with the primers OPF-01, P54, and 1253 for the isolates of , , and , respectively, showed the same polymorphic pattern as the reference strains for each species. To verify the specificity of the primers, they were tested with other species from the sections , and The results support that the primers OPF-01, P54, and 1253 generate polymorphic patterns by RAPD-PCR species specific to , , and , respectively.