Determination of Human Hemoglobin Derivatives

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly...

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Published inHemoglobin Vol. 39; no. 5; pp. 371 - 374
Main Authors Attia, Atef M. M., Ibrahim, Fatma A. A., Abd El-Latif, Noha A., Aziz, Samir W., Abdelmottaleb Moussa, Sherif A., Elalfy, Mohsen S.
Format Journal Article
LanguageEnglish
Published England Informa Healthcare 01.01.2015
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Summary:The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.
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ISSN:0363-0269
1532-432X
DOI:10.3109/03630269.2015.1062775