Stimulative effects of Ulmus davidiana Planch (Ulmaceae) on osteoblastic MC3T3-E1 cells

• Published in: Journal of ethnopharmacology Vol. 109; no. 3; pp. 480 - 485
• Main Authors: Suh, Seok-Jong, Yun, Woo-Sik, Kim, Kap-Sung, Jin, Un-Ho, Kim, June-Ki, Kim, Myung-Sunny, Kwon, Dae Young, Kim, Cheorl-Ho
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 12.02.2007; Elsevier
• Subjects: 3T3 Cells; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; anti-inflammatory activity; Biological and medical sciences; bone resorption; cell differentiation; cell growth; Cell Proliferation - drug effects; Cell Survival - drug effects; chemical constituents of plants; Dinoprostone - metabolism; Estrogen Antagonists - pharmacology; ethnobotany; General pharmacology; Herbal medicine; Medical sciences; medicinal plants; medicinal properties; Mice; Oriental traditional medicine; Osteoblastic MC3T3-E1 cell; osteoblasts; Osteoblasts - drug effects; Osteoblasts - metabolism; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Bark - chemistry; plant extracts; Plant Extracts - pharmacology; Procollagen-Proline Dioxygenase - metabolism; Prolyl hydroxylase; Prostaglandin E2; Rheumatoid arthritis; Tamoxifen - pharmacology; Ulmus - chemistry; Ulmus davidiana; Ulmus davidiana planch (Ulmaceae); PBS; Alkaline phosphatase; IL; ALP; Osteoblastic MC3T3-E1 cell; Prostaglandin E2; TNF; UD; RA; Herbal medicine; FBS; Rheumatoid arthritis; Ulmus davidiana planch (Ulmaceae); Prolyl hydroxylase; Diseases of the osteoarticular system; Phosphoric monoester hydrolases; Proline; Lipids; Autoimmune disease; Esterases; Inflammatory joint disease; Medicinal plant; Ulmus; Dicotyledones; Eicosanoid; Angiospermae; Immunopathology; Enzyme; Pharmacognosy; Arachidonic acid derivatives; Hydroxylase; Polyunsaturated fatty acid; Woody plant; In vitro; α-Aminoacid; Chronic; Aminoacid; Plant origin; Hydrolases; Unsaturated fatty acid; Spermatophyta; Oxidoreductases; Ulmaceae
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2006.08.030

Ulmus davidiana Planch (Ulmaceae) has long been known to have anti-inflammatory and protective effects on damaged tissue, inflammation and bone among other functions. To treat rheumatoid arthritis (RA), a herbal medicine, Ulmus davidiana Planch (Ulmaceae) extract (UD) is being used in traditional oriental medicine. The effect of UD on the proliferation and osteoblastic differentiation in non-transformed osteoblastic cells (MC3T3-E1) was studied. UD dose-dependently increased DNA synthesis (significant at 5–20 μg/ml). UD increased alkaline phosphatase (ALP) activity and prolyl hydroxylase activity of MC3T3-E1 cells (5–20 μg/ml). Antiestrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1, which was induced by UD. UD at concentrations ranged from 30 to 100 μg/ml inhibited prostaglandin E2 production in MC3T3-E1. These results indicate that UD directly stimulates cell proliferation and differentiation of osteoblasts. These results also suggest and UD is effective for bone anti-resorptive action in bone cells.