Nuclear deformation and cell division of single cell on elongated micropatterned substrates fabricated by DMD lithography

• Published in: Biofabrication Vol. 16; no. 3; pp. 35001 - 35022
• Main Authors: Zhang, Duo, Wu, Wenjie, Zhang, Wanying, Feng, Qiyu, Zhang, Qingchuan, Liang, Haiyi
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.07.2024
• Subjects: Cell Adhesion - physiology; Cell Division; cell geometry; DMD lithography; Elastomers; HeLa Cells; Humans; mechanotransduction; Mechanotransduction, Cellular; micropatterning; nuclear deformation; cell division; nuclear deformation; micropatterning; DMD lithography; mechanotransduction; cell geometry
• ISSN: 1758-5082; 1758-5090
• DOI: 10.1088/1758-5090/ad3319

Cells sense mechanical signals from the surrounding environment and transmit them to the nucleus through mechanotransduction to regulate cellular behavior. Microcontact printing, which utilizes elastomer stamps, is an effective method for simulating the cellular microenvironment and manipulating cell morphology. However, the conventional fabrication process of silicon masters and elastomer stamps requires complex procedures and specialized equipment, which restricts the widespread application of micropatterning in cell biology and hinders the investigation of the role of cell geometry in regulating cell behavior. In this study, we present an innovative method for convenient resin stamp microfabrication based on digital micromirror device planar lithography. Using this method, we generated a series of patterns ranging from millimeter to micrometer scales and validated their effectiveness in controlling adhesion at both collective and individual cell levels. Additionally, we investigated mechanotransduction and cell behavior on elongated micropatterned substrates. We then examined the effects of cell elongation on cytoskeleton organization, nuclear deformation, focal adhesion formation, traction force generation, nuclear mechanics, and the growth of HeLa cells. Our findings reveal a positive correlation between cell length and mechanotransduction. Interestingly, HeLa cells with moderate length exhibit the highest cell division and proliferation rates. These results highlight the regulatory role of cell elongation in mechanotransduction and its significant impact on cancer cell growth. Furthermore, our methodology for controlling cell adhesion holds the potential for addressing fundamental questions in both cell biology and biomedical engineering.