Genes expression of metalloproteinases (MMP-1, MMP-2, MMP-9, and MMP-12) associated with psoriasis

• Published in: Russian journal of genetics Vol. 47; no. 9; pp. 1117 - 1123
• Main Authors: Starodubtseva, N. L., Sobolev, V. V., Soboleva, A. G., Nikolaev, A. A., Bruskin, S. A.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.09.2011
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; Human Genetics; Microbial Genetics and Genomics; Psoriatic Lesion; Psoriasis; Primary Keratinocytes; Primary Human Keratinocytes; Psoriasis Patient
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S102279541109016X

Using real-time polymerase chain reaction (RT-PCR), we measured mRNA amounts of matrix metalloproteinases (MMPs): MMP-1, MMP-2, MMP-9 , and MMP-12 genes in psoriatic lesions and unaffected skin of the same patients. We observed significant (about 15-fold) increase in the expression level of matrix metalloproteinase MMP-1 and MMP-12 genes associated with psoriasis. The results of our studies of MMP gene expression in cultured primary human keratinocytes treated with interleukin (IL)-17 have shown upregulation of MMP gene expression both in cultured keratinocytes and in psoriatic skin lesions. Therefore, upregulation of MMP genes in the skin affected by psoriasis could result from IL-17 effects on skin cells.