Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 10; p. 2252
• Main Authors: Chu, Luan Luong, Tran, Chau T Bang, Pham, Duyen T Kieu, Nguyen, Hoa T An, Nguyen, Mi Ha, Pham, Nhung Mai, Nguyen, Anh T Van, Phan, Dung T, Do, Ha Minh, Nguyen, Quang Huy
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.05.2024
• Subjects: Acids; Antioxidants; Biosynthesis; Blood vessels; Cancer; Carbon; Chemical synthesis; Cocoa; Coronaviruses; Corynebacterium glutamicum - genetics; Corynebacterium glutamicum - metabolism; Corynebacterium glutamicum; Dengue fever; E coli; Engineering; Enzymes; flavonoid; Flavonoids; Flavonoids - biosynthesis; Flavonoids - metabolism; Functional foods & nutraceuticals; metabolic engineering; Metabolic Engineering - methods; Metabolism; Metabolites; Microorganisms; Physiology; secondary metabolites; Stilbenes - metabolism; stilbenoid; Corynebacterium glutamicum; stilbenoid; secondary metabolites; flavonoid; metabolic engineering
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29102252

Flavonoids and stilbenoids, crucial secondary metabolites abundant in plants and fungi, display diverse biological and pharmaceutical activities, including potent antioxidant, anti-inflammatory, and antimicrobial effects. However, conventional production methods, such as chemical synthesis and plant extraction, face challenges in sustainability and yield. Hence, there is a notable shift towards biological production using microorganisms like and yeast. Yet, the drawbacks of using and yeast as hosts for these compounds persist. For instance, yeast's complex glycosylation profile can lead to intricate protein production scenarios, including hyperglycosylation issues. Consequently, emerges as a promising alternative, given its adaptability and recent advances in metabolic engineering. Although extensively used in biotechnological applications, the potential production of flavonoid and stilbenoid in engineered remains largely untapped compared to . This review explores the potential of metabolic engineering in for biosynthesis, highlighting its versatility as a cell factory and assessing optimization strategies for these pathways. Additionally, various metabolic engineering methods, including genomic editing and biosensors, and cofactor regeneration are evaluated, with a focus on Through comprehensive discussion, the review offers insights into future perspectives in production, aiding researchers and industry professionals in the field.