Regulatory networks of the F-box protein FBX206 and OVATE family proteins modulate brassinosteroid biosynthesis to regulate grain size and yield in rice

• Published in: Journal of experimental botany Vol. 75; no. 3; pp. 789 - 801
• Main Authors: Sun, Xiaoxuan, Xie, Yonghong, Xu, Kaizun, Li, Jianxiong
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 02.02.2024
• Subjects: Brassinosteroids - metabolism; Edible Grain - genetics; Edible Grain - metabolism; F-Box Proteins - genetics; F-Box Proteins - metabolism; Gene Expression Regulation, Plant; Oryza - metabolism; Plant Growth Regulators - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Brassinosteroid; degradation; OVATE family protein; rice; ubiquitination; F-box protein
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/erad397

Abstract F-box proteins participate in the regulation of many processes, including cell division, development, and plant hormone responses. Brassinosteroids (BRs) regulate plant growth and development by activating core transcriptional and other multiple factors. In rice, OVATE family proteins (OFPs) participate in BR signalling and regulate grain size. Here we identified an F-box E3 ubiquitin ligase, FBX206, that acts as a negative factor in BR signalling and regulates grain size and yield in rice. Suppressed expression of FBX206 by RNAi leads to promoted plant growth and increased grain yield. Molecular analyses showed that the expression levels of BR biosynthetic genes were up-regulated, whereas those of BR catabolic genes were down-regulated in FBX206-RNAi plants, resulting in the accumulation of 28-homoBL, one of the bioactive BRs. FBX206 interacted with OsOFP8, a positive regulator in BR signalling, and OsOFP19, a negative regulator in BR signalling. SCFFBX206 mediated the degradation of OsOFP8 but suppressed OsOFP19 degradation. OsOFP8 interacted with OsOFP19, and the reciprocal regulation between OsOFP8 and OsOFP19 required the presence of FBX206. FBX206 itself was ubiquitinated and degraded, but interactions of OsOFP8 and OsOFP19 synergistically suppressed the degradation of FBX206. Genetic interactions indicated an additive effect between FBX206 and OsOFP8 and epistatic effects of OsOFP19 on FBX206 and OsOFP8. Our study reveals the regulatory networks of FBX206, OsOFP8, and OsOFP19 in BR signalling that regulate grain size and yield in rice. The F-box protein FBX206 and the OVATE family proteins form a regulatory network in the brassinosteroid signal pathway to modulate plant architecture, grain size, and grain yield.