Association of polymorphism of tumor necrosis factor-alpha gene promoter region with outcome of hepatitis B virus infection

• Published in: World journal of gastroenterology : WJG Vol. 11; no. 33; pp. 5213 - 5217
• Main Authors: Li, Hong-Quan, Li, Zhuo, Liu, Ying, Li, Jun-Hong, Dong, Jian-Qun, Gao, Ji-Rong, Gou, Chun-Yan, Li, Hui
• Format: Journal Article
• Language: English
• Published: United States School of Basic Medicine,Peking Union Medical College,Beijing 100005,China%Institute of Hepatitis Research,Beijing You'an Hospital,Capital Medical University,Beijing 100005,China 07.09.2005; Department of Epidemiology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences; Baishideng Publishing Group Inc
• Subjects: Adenine; Adult; Brief Reports; Case-Control Studies; Cytosine; Female; Genotype; Hepatitis B - genetics; Hepatitis B - physiopathology; Hepatitis B - virology; Hepatitis B virus - genetics; Hepatitis B, Chronic - genetics; Hepatitis B, Chronic - physiopathology; Humans; Male; Polymorphism, Genetic; Promoter Regions, Genetic - genetics; Thymine; Tumor Necrosis Factor-alpha - genetics; 乙型肝炎病毒; 基因多态性; 病毒感染; 肿瘤坏死因子-α; TNF-α gene; Genotype; Single nucleotide polymorphism; Haplotype; Hepatitis B
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i33.5213

AIM： To determine whether -238G/A and -857C/T polymorphisms of tumor necrosis factor-alpha （TNF-α）, gene promoter and hepatitis B （HB） viral genotypes were associated with outcomes of HBV infection. METHODS： A total of 244 HBV self-limited infected subjects, 208 asymptomatic carriers, and 443 chronic HB patients were recruited to conduct a case-control study. TNF-α -238G/A and -857C/T gene promoter polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, and HBV genotypes were examined by nested PCR. RESULTS： The positive rate of HBV DNA in asymptomatic carder group and chronic HB group was 46.6% and 49.9%, respectively. HBV genotype proportion among the asymptomatic carriers was 2.1% for genotype A, 25.8% for genotype B, 68.0% for genotype C, and 4.1% for genotype B＋C mixed infection, and 0.9% for genotype A, 21.7% for genotype B, 71.5% for genotype C, 5.9% for genotype B＋C mixed infection in chronic HB group. There was no significant difference in genotype distribution between the asymptomatic carrier group and chronic HB group （X^2 = 1.66, P = 0.647）. The frequency of -238GG genotype in self-limited group was 95.1%, significantly higher than 90.7% in chronic HB group and 89.0% in asymptomatic carrier group （P = 0.041 and P = 0.016, respectively）.The frequency of TNF-α-857 CC in chronic HB group was 79.7%, significantly higher than 64.4% in asymptomatic carrier group and 70.9% in self-limited group （P〈0.001 and P = 0.023, respectively）. A multiple logistic regression analysis revealed that TNF-α-238GA and -857CC were independently associated with chronic HB after gender and age were adjusted.CONCLUSION： TNF-α promoter variants are likely to play a substantial role in the outcome of HBV infection.