Distribution of the beta 1 subgroup of the integrins in human cells and tissues

We studied the distribution of the beta 1 integrin subfamily in human tissues and cells by light microscopy, electron microscopy, and immunoblotting, using monoclonal antibody DH12, previously shown to react with the beta 1 subunit of the human fibronectin receptor. Crossreaction with the other beta...

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Published inThe journal of histochemistry and cytochemistry Vol. 37; no. 3; pp. 299 - 307
Main Authors De Strooper, B, Van der Schueren, B, Jaspers, M, Saison, M, Spaepen, M, Van Leuven, F, Van den Berghe, H, Cassiman, JJ
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.03.1989
SAGE Publications
Subjects
Antibodies, Monoclonal
Electrophoresis, Polyacrylamide Gel
Female
Fibroblasts - analysis
Humans
Immunoblotting
Immunoenzyme Techniques
Immunosorbent Techniques
Integrins
Intestines - analysis
Kidney - analysis
Liver - analysis
Lung - analysis
Membrane Glycoproteins - analysis
Microscopy, Electron
Microscopy, Electron, Scanning
Placenta - analysis
Pregnancy
Skin - analysis
Spleen - analysis
Tissue Distribution
Umbilical Cord - analysis
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Summary:We studied the distribution of the beta 1 integrin subfamily in human tissues and cells by light microscopy, electron microscopy, and immunoblotting, using monoclonal antibody DH12, previously shown to react with the beta 1 subunit of the human fibronectin receptor. Crossreaction with the other beta subunits of the integrin family, which have 45% and 47% primary amino acid sequence identity with the beta 1 subunit, was excluded, as MAb DH12 did not react with the beta 2 subunit in granulocytes and the beta 3 subunit in thrombocytes. Reactivity with the anti-beta 1 antibody was found in skin, lung, heart, striated and smooth muscle, blood cells, liver, kidney, intestine, spleen and placenta. Thus, cells of mesodermal, ectodermal, and entodermal origin express the beta 1 subunit. In skin fibroblasts cultured in vitro, beta 1 subunit was also detected intracellularly. The wide distribution of the beta 1 family, originally detected in activated T-lymphocytes after prolonged culture in vitro, contrast with the restricted distribution of the beta 2 integrins on leucocytes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-1554
1551-5044
DOI:10.1177/37.3.2645360