Performance, nutrient utilization, and energy partitioning in broiler chickens offered high canola meal diets supplemented with multicomponent carbohydrase and mono-component protease

• Published in: Poultry science Vol. 96; no. 11; pp. 3960 - 3972
• Main Authors: Toghyani, M, Wu, S B, Pérez-Maldonado, R A, Iji, P A, Swick, R A
• Format: Journal Article
• Language: English
• Published: England 01.11.2017
• Subjects: Actinomycetales - chemistry; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; Aspergillus - chemistry; Bacillus licheniformis - genetics; Brassica - chemistry; Chickens - growth & development; Chickens - physiology; Diet - veterinary; Dietary Supplements - analysis; Digestion - drug effects; Energy Metabolism - drug effects; Fungal Proteins - administration & dosage; Fungal Proteins - metabolism; Glycoside Hydrolases - administration & dosage; Glycoside Hydrolases - metabolism; Male; Microorganisms, Genetically-Modified - genetics; Random Allocation; Serine Proteases - administration & dosage; Serine Proteases - metabolism; protease; canola meal; net energy; carbohydrase; energy partitioning
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps/pex212

Two broiler chicken experiments were conducted to investigate the effects of canola meal (CM) replacing soybean meal (SBM) in diets supplemented with carbohydrase and protease on performance and partitioning of energy. First, a 2 × 2 × 2 factorial arrangement of treatments was employed to evaluate: protein meals (CM vs. SBM), carbohydrase (none or 300 mg/kg), protease (none or 200 mg/kg), and their interactions. Each treatment was fed to 6 replicated pens of 16 male broilers (Ross 308) from d 10 to 35. In the second experiment, 32 broiler chicks were used in a 2 × 2 factorial arrangement to investigate CM and carbohydrase effects on energy partitioning. Birds were transferred into 16 closed-circuit calorimeter chambers (4 chambers/diet; 2 birds/chamber) to measure heat production (HP), metabolizable and net energy (NE) by gaseous exchange, and total excreta collection from d 25 to 28. There were no 3-way interactions among experimental factors for any of the performance parameters measured. Birds given CM diets consumed less feed, had lower BW, and exhibited higher FCR compared to the control birds (P < 0.01). Both enzymes, alone or in combination, improved final BW and FCR (P < 0.05). There was an interaction between carbohydrase and protease for FCR over the grower period (P < 0.01), in which the combination of the enzymes resulted in further improvement of FCR. Energy, DM, and crude protein digestibility values were higher in control birds (P < 0.05). There was an interaction of protein meal and carbohydrase for HP, respiratory quotient (P < 0.05), and NE:ME ratio of the diets (P = 0.06). Inclusion of CM without carbohydrase increased HP and decreased NE and NE:ME ratio of the diets (P < 0.05). Carbohydrase decreased HP and increased retained energy (P = 0.06) and NE and NE:ME ratio (P < 0.05). In conclusion, high CM in the diet negatively affects growth performance through reduction in feed consumption, nutrient digestibility, and NE of the diet, which could partly be restored by enzyme supplementation.