Identification of an L-dopa decarboxylase gene from Sorangium cellulosum So ce90

During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed...

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Published inArchives of microbiology Vol. 173; no. 4; pp. 303 - 306
Main Authors Müller, R, Gerth, K, Brandt, P, Blöcker, H, Beyer, S
Format Journal Article
LanguageEnglish
Published Germany 01.04.2000
Subjects
Amino Acid Sequence
ddc gene
Dopa Decarboxylase - chemistry
Dopa Decarboxylase - genetics
Dopa Decarboxylase - metabolism
Genes, Bacterial
l-Dihydroxy phenylalanine decarboxylase
Molecular Sequence Data
Myxococcales - enzymology
Myxococcales - genetics
Phenylalanine - metabolism
Phylogeny
Plasmids - genetics
Sequence Analysis, DNA
Sorangium cellulosum
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Summary:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed function derived from amino acid sequence comparisons. In contrast to other aromatic amino acid decarboxylases of eukaryotic origin, the S. cellulosum Ddc converted only L-dihydroxy phenylalanine. This is the first report of a gene encoding an L-dihydroxy phenylalanine decarboxylase in bacteria.
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ISSN:0302-8933
1432-072X
DOI:10.1007/s002039900131