Genomic diversity among Vibrio cholerae O139 strains isolated in Bangladesh and India between 1992 and 1998

• Published in: FEMS microbiology letters Vol. 184; no. 2; pp. 279 - 284
• Main Authors: Faruque, Shah M., Saha, Manujendra N., Asadulghani, Bag, Prasanta K., Bhadra, Rupak K., Bhattacharya, S.K., Sack, R.Bradley, Takeda, Yoshifumi, Nair, G.Balakrish
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2000; Blackwell; Oxford University Press
• Subjects: Antibiotic resistance; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Bacterial Typing Techniques; Bacteriology; Bangladesh - epidemiology; Biological and medical sciences; Cholera - epidemiology; Cholera - microbiology; Cholera Toxin - genetics; CTX genotype; DNA Restriction Enzymes; Drug resistance; Drug Resistance, Microbial; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genetic diversity; Genetic Variation; Genotypes; Humans; India - epidemiology; Microbial Sensitivity Tests; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pathogens; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Restriction Mapping; Ribotype; RNA, Ribosomal - genetics; Strains (organisms); Vibrio cholerae; Vibrio cholerae - classification; Vibrio cholerae - drug effects; Vibrio cholerae - genetics; Vibrio cholerae - isolation & purification; Vibrio cholerae O139; Waterborne diseases; Asia; Bangladesh; India; CTX genotype; Ribotype; Genetic diversity; O139; Antibiotic resistance; Human; Vibrio cholerae; Genotype; Characterization; Resistance; Antibiotic; Phenotype; Molecular epidemiology; Bacteria; Vibrionaceae; Antibacterial agent; Clinical isolate; Polymorphism
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2000.tb09027.x

Abstract In order to assess the extent of genomic diversity among Vibrio cholerae O139 strains, restriction fragment length polymorphisms in two genetic loci, rrn and ctx, were studied. Analysis of 144 strains isolated from different regions of Bangladesh and India between 1992 and 1998 revealed the presence of at least six distinct ribotypes (B-I through B-VI) of which three were new ribotypes, and one of these was represented by a nontoxigenic O139 strain. Strains of ribotypes B-I through B-V shared 11 different CTX genotypes (A through K). Antimicrobial resistance patterns of the strains varied independently of their ribotypes and CTX genotypes. Results of this study suggest that V. cholerae O139 is undergoing rapid genetic changes leading to the origination of new variants, and temporal changes in antimicrobial resistance patterns may be contributing to the selection of different variants.