Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound

Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke...

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Bibliographic Details
Published inCurrent protocols in neuroscience Vol. 93; no. 1; p. e98
Main Authors Kılıç, Kıvılcım, Tang, Jianbo, Erdener, Ş Evren, Sunil, Smrithi, Giblin, John T, Lee, Blaire S, Postnov, Dmitry D, Chen, Anderson, Boas, David A
Format Journal Article
LanguageEnglish
Published United States 01.09.2020
Subjects
Animals
Brain - diagnostic imaging
Functional Neuroimaging
Mice
Neurosciences - methods
Optical Imaging
Ultrasonography
functional ultrasound
mouse
chronic
imaging
optical
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Summary:Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Surgical preparation of mouse chronic cranial windows using polymethylpentene Basic Protocol 2: Imaging of mice with chronic cranial windows.
ISSN:1934-8576
DOI:10.1002/cpns.98