S100 and p65 expression are increased in the masseter muscle after botulinum toxin-A injection

• Published in: Maxillofacial plastic and reconstructive surgery Vol. 38; no. 1; p. 33
• Main Authors: Park, Young-Wook, Kim, Seong-Gon, Jo, You-Young
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 26.08.2016; 대한악안면성형재건외과학회
• Subjects: Dentistry; Medicine; Oral and Maxillofacial Surgery; Plastic Surgery; 치의학; S100; Masseter muscle; p65; Botulinum toxin-A; Apoptosis
• ISSN: 2288-8101; 2288-8586; 2288-8586
• DOI: 10.1186/s40902-016-0079-8

Background The purpose of this study was to compare the expression levels of p65 and S100 in the rat masseter muscle after the injection of different concentrations of botulinum toxin-A (BTX-A). Methods We injected either 5 or 10 U of BTX-A into both masseter muscle of rats. As a control group, the same volume of saline was injected. After 14 days, the animals were sacrificed. Subsequently, a biopsy and immunohistochemical staining of the samples were performed using a p65 or S100 antibody. Results The cross-sectional area of each myofibril was significantly reduced by BTX-A injection ( P  < 0.001). The expression of p65 and S100 increased significantly with increasing concentrations of BTX-A ( P  < 0.001). Conclusions The injection of BTX-A into the masseter muscle induced muscle atrophy. Subsequently, p65 and S100 expression in myoblasts were increased for the protection of muscle cells.