Cryptococcus neoformans Mating and Genetic Crosses

The Cryptococcus pathogenic species complex is a group of opportunistic human fungal pathogens that cause cryptococcal meningoencephalitis, an infection associated with unacceptably high mortality rates. The public health relevance of these pathogens has galvanized extensive research over the past s...

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Bibliographic Details
Published inCurrent protocols in microbiology (Online) Vol. 53; no. 1; p. e75
Main Authors Sun, Sheng, Priest, Shelby J, Heitman, Joseph
Format Journal Article
LanguageEnglish
Published United States 01.06.2019
Subjects
Crosses, Genetic
Cryptococcosis - microbiology
Cryptococcus neoformans - genetics
Cryptococcus neoformans - growth & development
Culture Media - chemistry
Culture Media - metabolism
Genes, Mating Type, Fungal
Genetic Techniques
Genotype
Humans
Mycology - methods
Spores, Fungal - genetics
Spores, Fungal - growth & development
mating progeny
Cryptococcus
genetic cross
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Summary:The Cryptococcus pathogenic species complex is a group of opportunistic human fungal pathogens that cause cryptococcal meningoencephalitis, an infection associated with unacceptably high mortality rates. The public health relevance of these pathogens has galvanized extensive research over the past several decades and led to characterization of their sexual cycles. This research has allowed several Cryptococcus species to develop into model fungal organisms for both pathogenesis and basic science studies. Many of these studies require observation of the meiotic process and its associated mating structures as well as generation of meiotic progeny with novel phenotypes and genotypes. Herein, we describe how to set up genetic crosses between Cryptococcus strains and observe their mating phenotypes as well as how to recover progeny from these crosses for further analysis. © 2019 by John Wiley & Sons, Inc.
ISSN:1934-8533
DOI:10.1002/cpmc.75