Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation

Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological...

Full description

Saved in:
Bibliographic Details
Published inCurrent protocols in microbiology (Online) Vol. 49; no. 1; p. e53
Main Authors Vellanki, Sandeep, Navarro-Mendoza, Maria Isabel, Garcia, Alexis, Murcia, Laura, Perez-Arques, Carlos, Garre, Victoriano, Nicolas, Francisco E, Lee, Soo Chan
Format Journal Article
LanguageEnglish
Published United States 01.05.2018
Subjects
Cloning, Molecular - methods
Colony Count, Microbial - methods
Culture Media - metabolism
Gene Silencing
Genetic Vectors - genetics
Genetic Vectors - metabolism
Humans
Mucor - genetics
Mucor - growth & development
Mucormycosis - microbiology
Polymerase Chain Reaction - methods
Preservation, Biological - methods
Sequence Deletion
Spores, Fungal - genetics
Spores, Fungal - growth & development
gene knockout
RNAi
Mucor
mucormycosis
Mucor circinelloides
Online AccessGet more information

Cover

More Information
Summary:Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference-mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.
ISSN:1934-8533
DOI:10.1002/cpmc.53