Fluorescence quenching and spectrophotometric methods for the determination of 6-mercaptopurine based on carbon dots

• Published in: RSC advances Vol. 6; no. 57; pp. 52255 - 52263
• Main Authors: Yuan, Yusheng, Wang, Yalan, Liu, Shaopu, Li, Yuanfang, Duan, Ruilin, Zhang, Hui, Hu, Xiaoli
• Format: Journal Article
• Language: English
• Published: 01.01.2016
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/c6ra07675k

It is of great significance to develop an eco-friendly and sensitive platform for the detection of 6-mercaptopurine (6-MP) because of its side effects and variable activity. Herein, fluorescence quenching and spectrophotometric methods for the detection of 6-mercaptopurine (6-MP) using carbon dots (CDs) as a fluorescence probe are established. 6-MP not only serves to shelter the CDs effectively from being quenched, but also to reverse the quenching and restore the fluorescence due to its ability to remove Hg 2+ from the surface of CDs. After the experimental conditions are optimized, the linear range for the detection of 6-MP is 0.04 to 12 μmol L −1 , and the detection limit is 0.01 μmol L −1 . When it refers to the spectrophotometric method, the linear range is 0.08 to 12 μmol L −1 and the detection limit is 0.02 μmol L −1 . The CDs with 6-MP are systematically characterized with transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) absorption and so on. Furthermore, the proposed methods are not only expected to become a potential tool for the fast response of 6-MP but also possess the potential for practical application to be applied in the determination of 6-MP in human serum samples with satisfactory results. A carbon dot-based fluorescence probe was designed for detecting 6-mercaptopurine (6-MP) via fluorescence quenching.