An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk

• Published in: Food analytical methods Vol. 12; no. 11; pp. 2430 - 2437
• Main Authors: Yang, Xingdong, Wang, Yinbiao, Yang, Jifei, Sun, Zhongke, Yue, Zonghao, Li, Lili, He, Le, Hu, Xiaofei
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2019; Springer Nature B.V
• Subjects: Analytical Chemistry; Assaying; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Coefficient of variation; Food Science; High performance liquid chromatography; Liquid chromatography; Microbiology; Milk; Monoclonal antibodies; Pasteurization; Strip; Danofloxacin; Monoclonal antibody; Colloidal gold; Immunochromatographic lateral flow strip test; Rapid and quantitative test
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-019-01601-9

An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a competitive format. Quantitative detections of DAN-spiked samples using the ILFST gave a half inhibitory concentration ( IC 50 ) of 0.513 ng/mL and a limit of detection (LOD) of 0.092 ng/mL. Recoveries of DAN from spiked milk samples were from 80.0 to 95.1% within an assay (intra-assay) and from 87.0 to 89.8% between assays (inter-assay). The coefficients of variation ( CV ) for intra-assay and inter-assay were 2.09–9.93% and 2.42–12.07%, respectively. These data indicated that the ILFST was highly sensitive and reproducible. Detections of authentic milk samples using ILFST and high-performance liquid chromatography (HPLC) showed no significant differences between the two methods. Hence, the ILFST can be widely used for both qualitative and quantitative detection of DAN residues in milk samples.