Expression of osa-miR7695 against the blast fungus Magnaporthe oryzae in Vietnamese rice cultivars

• Published in: European journal of plant pathology Vol. 155; no. 1; pp. 307 - 317
• Main Authors: Quoc, Nguyen Bao, Phuong, Nguyen Doan Nguyen, Trang, Ho Thi Thu, Phi, Nguyen Bang, Chau, Nguyen Ngoc Bao
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2019; Springer Nature B.V
• Subjects: Agriculture; Biomarkers; Biomedical and Life Sciences; Cultivars; Ecology; Fungi; Gene expression; Immunity; Inoculation; Life Sciences; Magnaporthe oryzae; MicroRNAs; miRNA; Oryza sativa indica; Plant Pathology; Plant Sciences; Polymerase chain reaction; Ribonucleic acid; Rice; Rice blast; RNA; Vietnam; qRT-PCR
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-019-01772-5

Magnaporthe oryzae causes rice blast disease, which leads to devastating yield losses in Vietnam and other rice-growing countries. Recent studies indicated that 30% of rice- growing areas are affected by rice blast disease. MicroRNAs (miRNAs) are short non-coding RNAs that are related to plant development and biotic stress tolerance. Osa-miR7695 as one of the common miRNAs, and its target the OsNramp6 gene were evaluated as factors that contribute to the pathogenic activity of different rice cultivars . The expression profile of microRNA osa-miR7695 was evaluated to identify blast resistance or susceptibility in rice cultivars. Results of RT-PCR and real-time qPCR indicated the presence of osa-miR7695 in most Oryzae sativa Indica group cultivars in Vietnam. Upregulation of osa-miR7695 observed in blast resistant rice cultivars at different infection time intervals was higher than in blast non-resistant rice cultivars. Expression of the OsNramp6 gene increased in blast non-resistant cultivars, particularly at 72 h post inoculation. Average ΔCt values of osa-miR7695 in blast resistant rice cultivars were higher by approximately 4-fold than in blast non-resistant rice cultivars. Results suggested osa-miR7695 as a potential biomarker for microRNA based detection of blast resistance and non-resistance in Vietnamese rice cultivars to improve understanding of the biological role of microRNA in rice immunity.