Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties
The translation initiation factor 4E (eIF4E) has been implicated in naturally-occurring resistance to the Potato Virus Y (PVY) determined by the pot-1 locus in tomato. The susceptible potato varieties Russet Norkotah, Silverton Russet, and Classic Russet, along with the Michigan State University bre...
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Published in | American journal of potato research Vol. 98; no. 1; pp. 42 - 50 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.02.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The translation initiation factor 4E (eIF4E) has been implicated in naturally-occurring resistance to the
Potato Virus Y
(PVY) determined by the
pot-1
locus in tomato. The susceptible potato varieties Russet Norkotah, Silverton Russet, and Classic Russet, along with the Michigan State University breeding line MSE149-5Y, were transformed with the
pot-1
gene from
Solanum hirsutum
to achieve PVY resistance. Transgenic potato events and their non-transgenic controls were screened for PVY resistance against three PVY strains (PVY
O
, PVY
N:O
, PVY
NTN
) by enzyme-linked immunosorbent assay (ELISA) in greenhouse and in field trials. The event A95.87.05 (Classic Russet) and MSE149.87.22 (MSE149-5Y) had resistance against all three PVY isolates tested in both foliage and tuber under either greenhouse or field conditions. In contrast, SR.87.62 (Silverton Russet) and RN1.87.09 (Russet Norkotah), displayed moderate susceptibility to PVY in foliage during the growing season and increased level of infection in tubers. Transformation of PVY-susceptible potato varieties with the tomato
pot-1
gene can be a useful tool for improving PVY resistance. |
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ISSN: | 1099-209X 1874-9380 |
DOI: | 10.1007/s12230-020-09815-y |