Production Conditions for Bruceanol B, an Antibiotic that Generates Oxygen Radical

• Published in: Applied biological chemistry Vol. 54; no. 1; pp. 128 - 131
• Main Authors: Lee, D.S., Jeju National University, Jeju, Republic of Korea, Boo, K.H., Jeju National University, Jeju, Republic of Korea, Woo, J.K., Jeju National University, Jeju, Republic of Korea, Jeon, G.L., Jeju National University, Jeju, Republic of Korea, Lee, J.M., Hoseo University, Asan, Republic of Korea, Lee, S.H., Kyungpook National University, Daegu, Republic of Korea, Kim, J.H., Jeju National University, Jeju, Republic of Korea, Riu, Key Zung, Jeju National University, Jeju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.02.2011; Springer Nature B.V; 한국응용생명화학회
• Subjects: Acetic acid; Antibacterial activity; Antibacterial agents; Antibiotics; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; bruceanol B; Chemistry; Chemistry and Materials Science; Ethyl acetate; High performance liquid chromatography; HPLC; Liquid chromatography; production condition; radical-generating antibiotic; Reactive oxygen species; Short Communication; Silica; Silica gel; Silicon dioxide; Soil microorganisms; Starch; Yeast; Yeasts; 농학; production condition; bruceanol B; radical-generating antibiotic
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.3839/jksabc.2011.019

Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30℃ for 3.5 days.