Genetic Analysis and Gene Mapping of Short Root Mutant Rice ksr1

A short root mutant ksrl with the Kasalath background was isolated from an EMS-mutagenized population in rice. The root length of 6-day-old ksr1 seedlings was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksrl was controlled by a recessive mutation in a...

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Bibliographic Details
Published inRice science Vol. 18; no. 1; pp. 67 - 70
Main Authors NING, Yong-qiang, DING, Wo-na, ZHU, Shi-hua, YU, Hang-wei, YU, Hang, LU, Kai-xing
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2011
Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China
Subjects
4号染色体
EMS诱变
gene mapping
molecular marker
rice
short root
SSR标记
基因定位
水稻
短根突变体
编码基因
遗传分析
short root
rice
gene mapping
molecular marker
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Summary:A short root mutant ksrl with the Kasalath background was isolated from an EMS-mutagenized population in rice. The root length of 6-day-old ksr1 seedlings was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksrl was controlled by a recessive mutation in a single nuclear-encoded gene. To map the ksrl mutation, an F2 population was generated by crossing the ksrl mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on rice chromosome 4. Eight new SSR markers and two InDel markers were developed around this marker. KSR1 gene was further mapped to a 155 kb region, flanked by the InDel marker 4-24725K and the SSR marker RM17182.
Bibliography:33-1317/S
rice; short root; gene mapping; molecular marker
A short root mutant ksrl with the Kasalath background was isolated from an EMS-mutagenized population in rice. The root length of 6-day-old ksr1 seedlings was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksrl was controlled by a recessive mutation in a single nuclear-encoded gene. To map the ksrl mutation, an F2 population was generated by crossing the ksrl mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on rice chromosome 4. Eight new SSR markers and two InDel markers were developed around this marker. KSR1 gene was further mapped to a 155 kb region, flanked by the InDel marker 4-24725K and the SSR marker RM17182.
ISSN:1672-6308
1876-4762
DOI:10.1016/S1672-6308(11)60009-7