Alternative Nitrogen Source for Producing Crude Extracted IAA and Suitable Method for Enhancing the Germination of Jerusalem Artichoke

• Published in: Waste and biomass valorization Vol. 14; no. 5; pp. 1497 - 1508
• Main Authors: Onrit, Natnicha, Boonlue, Sophon, Mongkolthanaruk, Wiyada, Jogloy, Sanun, Riddech, Nuntavun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2023; Springer Nature B.V
• Subjects: Acetic acid; Casein; Crop residues; Dipping; Elongation; Engineering; Environment; Environmental Engineering/Biotechnology; Germination; Helianthus tuberosus; Indoleacetic acid; Industrial Pollution Prevention; Jerusalem artichokes; Nitrogen; Nitrogen sources; Original Paper; Plant roots; Poultry manure; Renewable and Green Energy; Residues; Seedlings; Shoots; Soybeans; Tryptophan; Waste Management/Waste Technology; Yeasts; Jerusalem Israel; Israel; L.; Indole-3-acetic acid (IAA); Jerusalem artichoke; Soybean residue
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-022-01970-z

Auxin is a useful compound which has an effect on plant root and shoot elongation. Thus, the aim of this study was to determine a suitable medium formula to produce indole-3-acetic acid (IAA). Adding various nitrogen sources such as beef extract, chicken manure solution, soybean residue, yeast extract, soybean powder and casein, supplemented with and without inducible substances such as tryptophan, optimized incubation temperature. IAA content in crude extraction was detected by TLC chromatography. Moreover, both the optimum concentration of IAA crude and the most suitable method (spreading or dipping) for application on Jerusalem artichoke were investigated. Five isolates revealed positive results on IAA product. The alternative medium for IAA production in the formula that consisted of soybean residue showed significantly highest contents of substances like IAA product when the rhizobacteria was cultured at 25 and 30 °C. Sixty µg/ml of crude extracted IAA was the optimum concentration, promoting Jerusalem artichoke ( Helianthus tuberosus L.), growth with 100% germination. In addition, the root and shoot lengths exhibited were 3.79 and 7.14 cm, respectively. Dipping seed method was the most effective approach for encouraging seedling development, with a percentage of germination of 86.67 and root and shoot lengths of 4.48 and 5.39 cm, respectively. Our results demonstrated that a simple approach and appropriate concentration of crude extracted IAA could boost Jerusalem artichoke seedling development. Graphical Abstract