Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus)
Myrtle rust (caused by Austropuccinia psidii Beenken) is exotic to Australia, yet specific resistance is present in a range of naïve myrtaceous host plants. Resistance to myrtle rust is primarily quantitative in nature and controlled by multiple interacting loci. We undertook a genome-wide associati...
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Published in | Tree genetics & genomes Vol. 17; no. 3 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.06.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Myrtle rust (caused by
Austropuccinia psidii
Beenken) is exotic to Australia, yet specific resistance is present in a range of naïve myrtaceous host plants. Resistance to myrtle rust is primarily quantitative in nature and controlled by multiple interacting loci. We undertook a genome-wide association study (GWAS) to discover relationships between nuclear genomic sequence variation and multiple aspects of resistance to
A. psidii
in
Eucalyptus obliqua
, a representative species from subgenus
Eucalyptus
. Sequence variation was assessed with respect to numeric severity, binary symptomatic, hypersensitive, and pustulation responses to inoculation of seedlings with
A. psidii
. A total of 1.13 million, single-nucleotide polymorphisms (SNPs) were tested for association with the rust resistance responses of 637 phenotyped
E. obliqua
seedlings, each from different, single mother tree seedlots. Thirty-three highly significant SNP-trait associations were detected, of which 26 associated with the binary symptomatic/asymptomatic response to
A. psidii
. Comparison of the genomic position of these SNPs with rust resistance loci (
Ppr1-5
), previously reported in species from subgenus
Symphyomyrtus
, suggested that several were positioned near the major
Ppr1
locus and other
Ppr
loci. This study provides the first integrated genomic view of
A. psidii
resistance across the
Eucalyptus
subgenera and provides the foundation for discovering key resistance genes for use in marker-based resistance breeding. |
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ISSN: | 1614-2942 1614-2950 |
DOI: | 10.1007/s11295-021-01511-0 |