Optimization of Purpureocillium lilacinum Derived from Spent Mushroom Substrate and Its Potential to Control Soybean Cyst Nematode

• Published in: Waste and biomass valorization Vol. 14; no. 5; pp. 1637 - 1647
• Main Authors: Gao, Feng, Yuan, Yafeng, Yu, Xiaobin, Jiang, Qiushi, Zhang, Zhongkai, Hu, Xueqing, Zhao, Chunli, Cheng, Zhiqiang
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2023; Springer Nature B.V
• Subjects: Engineering; Environment; Environmental Engineering/Biotechnology; Fermentation; Granular materials; Industrial Pollution Prevention; Inoculum; Mushrooms; Optimization; Original Paper; Pesticides; Plant growth; Purpureocillium lilacinum; Renewable and Green Energy; Soybeans; Spores; Substrates; Waste Management/Waste Technology; Zinc chloride; Spent mushroom substrate; Bio-pesticides; Soybean cyst nematode
• ISSN: 1877-2641; 1877-265X
• DOI: 10.1007/s12649-022-01930-7

The spent mushroom substrate is usually wasted after culturing, causing environmental pollution and waste of resources. In this study, spent mushroom substrate was used as the source to cultivate Purpureocillium lilacinum . Under optimal conditions (SMS: water ratio was 1:6, 0.5% ZnCl 2 , 1% NaNO 3 , 7% inoculum volume, 29 °C, and 6 days), the fermentation product of P. lilacinum was obtained (1.18 × 10 8 spores/mL). Granules with a diameter of 0.2–0.5 cm and a live fungi content of 1.3 × 10 7  CFU/g were prepared by using spent mushroom substrate and the fermentation product of P. lilacinum. The pot experiments were carried out to evaluate the control effect of the granules on soybean cyst nematode. The results of the pot experiment showed that the application of granules could reduce the cysts by 80.1% and promote the growth of plants. The granules can be stored at 4 °C for more than 6 months. The results proved the feasibility of preparing bio-pesticides from the spent mushroom substrate. Graphical abstract