Three-dimensional magnetic enzyme-inorganic hybrid nanocomplexes with high reusability and stability to obtain lactose-free products

• Published in: Chemical papers Vol. 75; no. 10; pp. 5353 - 5362
• Main Authors: Yi, Fei, Liu, Yu-Cheng, Yang, Yun-Jia, Ji, Xing-Hu, He, Zhi-Ke
• Format: Journal Article
• Language: English
• Published: Warsaw Versita 01.10.2021; Springer Nature B.V
• Subjects: Biochemistry; Biotechnology; Calcium phosphates; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Dimensional stability; Galactosidase; Hydrolysis; Industrial Chemistry/Chemical Engineering; Iron oxides; Lactose; Low cost; Materials Science; Medicinal Chemistry; Nanoparticles; Original Paper; Immobilization; Enzyme reaction; Magnetic β-galactosidase hybrid nanocomplexes; Magnetic separation; Lactose hydrolysis
• ISSN: 0366-6352; 1336-9075; 2585-7290
• DOI: 10.1007/s11696-021-01726-4

Multitudinous people suffer from lactose intolerance which is bound up with lactose malabsorption. Enzymatic hydrolysis is the most common approach for the preparation of lactose-free products. The recycling of hydrolytic enzymes is an effective way to produce low-cost lactose-free products. Herein, a facile strategy for the preparation of the novel Fe 3 O 4 magnetic nanoparticles-β-galactosidase-CaHPO 4 hybrid nanocomplexes (β-gal MNCs) was established. β-gal MNCs were composed of amino-modified Fe 3 O 4 magnetic nanoparticles, β-galactosidase and crystalline calcium hydrogen phosphates. Meanwhile, under the external magnetic field, the magnetic β-gal MNCs can be easily and rapidly separated from the reaction medium. In addition, the β-galactosidase maintained most of its initial activity after immobilization into the β-gal MNCs. In a continuous hydrolysis reaction, β-gal MNCs exhibited excellent reusability and good stability. Therefore, β-gal MNCs hold promising prospects in the fast and low-cost preparation of lactose-free milk.