Portable in situ fluorescence cytometry of microscale cell-based assays

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (microCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to...

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Bibliographic Details
Published inOptics letters Vol. 30; no. 13; p. 1689
Main Authors Tatosian, Daniel A, Shuler, Michael L, Kim, Donghyun
Format Journal Article
LanguageEnglish
Published United States 01.07.2005
Subjects
Animals
Biological Assay - instrumentation
Biological Assay - methods
Cell Line
Daunorubicin - pharmacokinetics
Equipment Design
Equipment Failure Analysis
Feasibility Studies
Fibroblasts - metabolism
Flow Cytometry - instrumentation
Flow Cytometry - methods
Mice
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Miniaturization
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Summary:A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (microCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a microCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the microCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.
ISSN:0146-9592
DOI:10.1364/OL.30.001689