Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress

• Published in: Sugar tech : an international journal of sugar crops & related industries Vol. 24; no. 6; pp. 1680 - 1688
• Main Authors: Chen, Jiao-Yun, Sun, Bo, Khan, Qaisar, Yang, Li-Tao, Zhang, Bao-Qing, Xing, Yong-Xiu, Li, Yang-Rui
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.12.2022; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Catalase; Cold tolerance; Fluorescence; Gene expression; Hybrids; Life Sciences; Low temperature; Peroxidase; Polymerase chain reaction; Proteins; Research Article; Saccharum; Stress; Sugarcane; Superoxide dismutase; Temperature tolerance; Tissue analysis; Tobacco; Transgenic plants; Tubulin; SoTUA; Tobacco; Transformation; Cold stress; Sugarcane
• ISSN: 0972-1525; 0974-0740
• DOI: 10.1007/s12355-022-01118-2

Low temperature stress affects the yield and quality of crops. To adapt to abiotic stress, plant tubulin families play a crucial role to increase cell cold stability. In the present study, our aim was to develop transgenic tobacco by using a cold-responsive SoTUA gene isolated from Saccharum spp. hybrid. Based on fluorescent microscope observation and TUA gene expression analysis in different tissues, TUA gene expression was found mainly in veins. Moreover, a significant increase of TUA transcripts and protein under cold stress was detected by quantificational real-time polymerase chain reaction (qRT-PCR) and western blot in the transgenic plants. Results of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activities, and proline, soluble sugar and protein contents indicated oxidative damage is less than wild type (WT). Overexpression of SoTUA gene has improved the cold stress tolerance ability in tobacco.