Diversity of Botryosphaeriaceae causing grapevine trunk diseases and their spatial distribution under different climatic conditions in Algeria

• Published in: European journal of plant pathology Vol. 161; no. 4; pp. 933 - 952
• Main Authors: Arkam, Mounia, Alves, Artur, Lopes, Anabela, Čechová, Jana, Pokluda, Robert, Eichmeier, Aleš, Zitouni, Abdelghani, Mahamedi, Alla Eddine, Berraf-Tebbal, Akila
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2021; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Botryosphaeria; Botryosphaeriaceae; Climate; Climatic conditions; Deoxyribonucleic acid; Deserts; DNA; Ecological conditions; Ecology; Elongation; Endophytes; Fungi; Gene sequencing; Grapevines; Horticulture; Life Sciences; Nucleotide sequence; Pathogenicity; Pathogens; Plant Pathology; Plant Sciences; Shoots; Spatial distribution; Species; Translation elongation; Vineyards; Wineries & vineyards; Wines; Algeria; Pathogenicity; Botryosphaeria dieback; Taxonomy; Climatic conditions; Grapevine
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-021-02377-7

The family Botryosphaeriaceae is one of the most widespread and cosmopolitan endophytic group of fungi. Every year, species of this family cause severe damages on table and wine grape production, worldwide. However, this threat is still poorly known in Algeria. In this study, a large number of Botryosphaeriaceae -like isolates were obtained from symptomatic grapevines collected from eight regions with different ecological conditions, namely: Boumerdès, Médéa, Algiers, Tipaza, El Taref, Sidi Bel Abbes, Biskra and Adrar. The isolates were identified using DNA sequences of the translation elongation factor ( tef1-α ) and internal transcribed spacer (ITS) regions. Eleven species belonging to six genera, including Neofusicoccum parvum , N. algeriense , N. vitifusiforme , N. stellenboschiana , N. luteum , Diplodia seriata , D. olivarum , Lasiodiplodia theobromae , Dothiorella sarmentorum , Botryosphaeria dothidea and Neoscytalidium dimidiatum were identified. The spatial distribution of the Botryosphaeriaceae showed that D. seriata and N. stellenboschiana were the most widespread in the Algerian vineyards, while L. theobromae was recorded in the desert region of Biskra. Pathogenicity trials showed that all species were pathogenic on detached green shoots of grapevine, with N. parvum and L. theobromae being the most aggressive.