Benign Synthesis of Cobalt Oxide Nanoparticles Containing Red Algae Extract: Antioxidant, Antimicrobial, Anticancer, and Anticoagulant Activity

• Published in: Journal of cluster science Vol. 33; no. 2; pp. 717 - 728
• Main Authors: Ajarem, Jamaan S., Maodaa, Saleh N., Allam, Ahmed A., Taher, Mohamed M., Khalaf, Mohamed
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer Nature B.V
• Subjects: Algae; Anticancer properties; Anticoagulants; Antioxidants; Bacteria; Biological properties; Biosynthesis; Cancer; Catalysis; Chemicals; Chemistry; Chemistry and Materials Science; Cobalt; Cobalt oxides; Diameters; Dilution; Free radicals; Gram-positive bacteria; Inorganic Chemistry; Metal oxides; Morphology; Nanochemistry; Nanoparticles; Nanotechnology; Nitrates; Original Paper; Oxytetracycline; Physical Chemistry; Scanning electron microscopy; Scavenging; Synthesis; Japan; Anticoagulant and thrombolytic property; Antibacterial; Anticancer; Cobalt nanoparticles
• ISSN: 1040-7278; 1572-8862
• DOI: 10.1007/s10876-021-02004-9

Eco-friendly synthesis of Cobalt oxide nanoparticles (Co 3 O 4 NPs) was assessed by the aqueous extract of red algae and their utilization as cytotoxicity, antioxidant, anticoagulant, antibacterial, and anti-cancer properties. UV–vis, SEM, TEM, FTIR, EDAX, XRD, and TGA are tools used for characterization purposes. The size of the Co 3 O 4 NPs crystal of 28.2 ± 7.8 nm was measured based on the X-ray diffraction analysis. Biosynthesized Co 3 O 4 NPs showed uniform spherical morphology with an average diameter of 29.8 ± 8.6 nm according to TEM and SEM images. Furthermore, Co 3 O 4 NPs biological properties such as determination of the antibacterial properties of Co 3 O 4 NPs by the zone of inhibition method and the minimum inhibitory concentration (MIC) were determined using a dilution broth agar test. Co 3 O 4 NPs indicated higher antibacterial than oxytetracycline standard antibiotics. On the other hand, a scavenging free radical DPPH study was done to determine the propensity for antioxidants that showed a similar capacity for antioxidants in Co 3 O 4 NPs. The biosynthesized Co 3 O 4 NPs are effective erythrocyte viability dose-dependently and indicated this method was nontoxic. Moreover, bioinspired Co 3 O 4 NPs has promising results against HepG2 cancer cells (IC50: 41.4 μg/ml). Most notably, biogenic Co 3 O 4 NPs have anticoagulant and thrombolytic activities by the addition of nanoparticles to human blood samples under practical conditions. According to these context findings, Co 3 O 4 NPs would be used as a medicinal aid along with its anti-cytotoxic, antioxidant, and effects on bacteria, and cancer.