High frequency production of hybridomas secreting antibodies to cell antigens

The efficiency of various immunization protocols for the production of hybridomas secreting immunoglobulins specific to cell antigens was evaluated in 15 different fusion experiments. Some of these experiments were performed with splenic B-lymphocytes from mice at different stages of immunization. T...

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Bibliographic Details
Published inHybridoma Vol. 6; no. 6; p. 673
Main Authors Cianfriglia, M, Nuti, M, Turchi, V, Barca, S, Tombesi, M, Morrone, S, Cenciarelli, C, Natali, P G
Format Journal Article
LanguageEnglish
Published United States 01.12.1987
Subjects
Animals
Antibodies, Monoclonal - biosynthesis
Antigens - immunology
B-Lymphocytes - immunology
Cell Fusion
Humans
Hybridomas - immunology
Immunization
Mice
Spleen - immunology
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Summary:The efficiency of various immunization protocols for the production of hybridomas secreting immunoglobulins specific to cell antigens was evaluated in 15 different fusion experiments. Some of these experiments were performed with splenic B-lymphocytes from mice at different stages of immunization. This approach allowed a dynamic analysis of immunocompetent splenic B-lymphocyte production during the immunization cycle.
ISSN:0272-457X
DOI:10.1089/hyb.1987.6.673