Rudaibacter terrae gen. nov., sp. nov., isolated from greenhouse soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 63; no. Pt 11; pp. 4052 - 4057
• Main Authors: Kim, Soo-Jin, Moon, Ji-Young, Hamada, Moriyuki, Tamura, Tomohiko, Weon, Hang-Yeon, Suzuki, Ken-ichiro, Kwon, Soon-Wo
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.11.2013
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Aminobutyrates - chemistry; bacteria; Bacterial Typing Techniques; Base Composition; Cell Wall - chemistry; DNA, Bacterial - genetics; Environment, Controlled; fatty acids; Fatty Acids - chemistry; glycolipids; greenhouse soils; Herbiconiux; Leifsonia aquatica; Leifsonia poae; menaquinones; Molecular Sequence Data; new genus; new species; nucleotide sequences; Peptidoglycan - chemistry; peptidoglycans; Phenotype; Phylogeny; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; sodium chloride; Soil Microbiology; temperature; Vitamin K 2 - chemistry; South Korea
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.049817-0

A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4T, was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10–37 °C (optimum 28–30 °C) and at pH 5.0–9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1–97.0 % with species of the genus Leifsonia, 95.7–96.7 % with species of the genus Herbiconiux, 95.1–96.4 % with species of the genus Salinibacterium and 96.1 % with Labedella gwakjiensis and Homoserinimonas aerilata. The highest sequence similarities (97.0 %) were with Leifsonia aquatica JCM 1368T, Leifsonia poae VKM Ac-1401T and Leifsonia psychrotolerans LI1T. The peptidoglycan type determined for strain 5GHs34-4T was B2γ with dl-2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), anteiso-C17 : 0 and anteiso-C15 : 0. The phenotypic and phylogenetic traits of strain 5GHs34-4T differed in some respects from those of members of the family Microbacteriaceae. Therefore, strain 5GHs34-4T is considered to represent a novel species of a new genus in the family Microbacteriaceae, for which the name Rudaibacter terrae gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4T ( = KACC 15523T = NBRC 108754T).