Clindamycin-loaded nanofibers of polylactic acid, elastin and gelatin for use in tissue engineering

• Published in: Polymer bulletin (Berlin, Germany) Vol. 79; no. 7; pp. 5495 - 5513
• Main Authors: Castillo-Ortega, M. M., López-Peña, I. Y., Rodríguez-Félix, D. E., Del Castillo-Castro, T., Encinas-Encinas, J. C., Santacruz-Ortega, H., Cauich-Rodríguez, J. V., Quiroz-Castillo, J. M., Chan-Chan, L. H., Lagarda-Diaz, I., Plascencia-Martínez, D. F., Leyva-Verduzco, A. A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2022; Springer Nature B.V
• Subjects: Aluminum; Biocompatibility; Cell adhesion; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Diabetes; Disease prevention; Elastin; Electron microscopes; Extracellular matrix; Fibers; Fourier transforms; Gelatin; Gram-negative bacteria; Organic Chemistry; Original Paper; Physical Chemistry; Polylactic acid; Polymer Sciences; Polymers; Skin; Soft and Granular Matter; Solvents; Spectrum analysis; Thermal analysis; Thermogravimetric analysis; Tissue engineering; Transmission electron microscopy
• ISSN: 0170-0839; 1436-2449
• DOI: 10.1007/s00289-021-03734-6

In the present work, polylactic acid (PLA), elastin and gelatin fibers, containing clindamycin, were prepared to test their potential application as wound dressings. They underwent release studies to determine the mechanism of drug release through mathematical models. The fibers have a homogeneous morphology, without pores. The studies of FTIR and thermal analysis corroborate the presence of each of the components in the fibers; the results of the feasibility tests showed encouraging percentages with a viability of 82% after 7 days of direct contact of the HUVEC cells with the membranes. Similarly, cell adhesion assays show the presence of viable and stretched cells on the fibers, the tendency of the cells to position themselves on the fibers and follow this conformation was observed. On the other hand, studies of antimicrobial activity against S. aureus show us that in fibers loaded with clindamycin they have inhibition halos greater than 8 mm.