Valorization of mangaba residue (Hancornia speciosa Gomes) for polygalacturonase production from Aspergillus niger IOC 4003 and fabrication of active chitosan films

• Published in: Biomass conversion and biorefinery Vol. 12; no. 9; pp. 4069 - 4080
• Main Authors: de Medeiros Bezerra Jácome, Millena Cristiane, de Araújo Padilha, Carlos Eduardo, do Nascimento Arrais, Murilo Ricardo, de Sá Leitão, Ana Laura Oliveira, de Sousa Júnior, Francisco Canindé, dos Santos, Everaldo Silvino
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2022; Springer Nature B.V
• Subjects: Antioxidants; Biotechnology; Chitosan; Energy; Enzymes; Lignin; Original Article; Polygalacturonase; Reduction; Renewable and Green Energy; Residues; Sodium hydroxide; Solvent extraction; Substrates; Turbidity; Lignin; Fractionation; Juice treatment; Antioxidant activity; Solid state fermentation
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-020-01102-4

The present study proposes to obtain polygalacturonases, antioxidant compounds, and active chitosan films from a valorization scheme of mangaba residue. After extraction with ethanolic solution, the mangaba residue was used as a substrate for solid state fermentation (SSF) of Aspergillus niger IOC 4003, which obtained 76.32 U g −1 for polygalacturonase after 96 h cultivation. The previous step of solvent extraction increased the enzyme productivity and allowed the production of pH-tolerant polygalacturonase in SSF experiments. The addition of polygalacturosanase under 1.0 U mL −1 enabled the reduction of turbidity (34%) and viscosity (11%) of mangaba juice compared to enzyme-free condition. Solid residue from the SSF step was used in the extraction of alkaline lignin with sodium hydroxide, which allowed the recovery of 21.82% of the initial Klason lignin content. Supplementation of chitosan films with ethanolic extract and 0.1% (w v −1 ) alkaline lignin changed the values of antioxidant activity (increase of 86.5% and 45.4%) and permeability (reduction of 43.2% and 2.3%).