Similarities of the Golgi apparatus membrane and the plasma membrane in rat liver cells

• Published in: Journal of cell science Vol. 20; no. 1; pp. 167 - 182
• Main Authors: Hodson, S, Brenchley, G
• Format: Journal Article
• Language: English
• Published: England 01.01.1976
• Subjects: Animals; Cell Membrane - analysis; Cell Membrane - enzymology; Cell Membrane - ultrastructure; Electron Transport Complex IV - metabolism; Galactosyltransferases - metabolism; Glucose-6-Phosphatase - metabolism; Golgi Apparatus - analysis; Golgi Apparatus - enzymology; Golgi Apparatus - ultrastructure; Liver - ultrastructure; Male; Membranes - ultrastructure; Molecular Weight; Nucleotidases - metabolism; Proteins - analysis; Rats; Subcellular Fractions - enzymology; Subcellular Fractions - ultrastructure
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.20.1.167

A Golgi apparatus-rich fraction and a plasma membrane-rich fraction were isolated from a common homogenate of rat liver. Their respective buovant densities, appearances in the electron microscope and 5'-nucleotidase and UDP-galactose ovalbumin galactosyltransferase activities were in accord with published data on separately isolated Golgi apparatus-rich and plasma membrane-rich fractions. Contamination by endoplasmic reticulum and mitochondria was low. Gel electrophoresis of the membrane proteins of the Golgi apparatus-rich and plasma membrane-rich fractions (separately and mixed) showed a close similarity. After Neville's demonstration that electrophoretic patterns of membrane protein subunits from different subcellular fractions are easily distinguishable, the present work demonstrates an unusually close relationship between the Golgi apparatus membrane and the cell membrane. It is possible that membrane similarity may be mediated by the transfer of membrane-bound vesicles from the Golgi apparatus to the cell membrane.