Heterologous expression of Sesuvium portulacastrum SOS-related genes confer salt tolerance in yeast

• Published in: Acta physiologiae plantarum Vol. 45; no. 4
• Main Authors: Zhou, Yang, Zhu, Yunfeng, Li, Wei, Zhang, Tingting, Li, Yuxin, Kang, Yuqian, Wang, Jian, Guo, Jianchun, Jiang, Xingyu
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2023; Springer Nature B.V
• Subjects: Abiotic stress; Agriculture; Amino acids; Biomedical and Life Sciences; C-Terminus; Calcineurin; Cbl protein; Cell membranes; Genes; Halophytes; Hydrogen; Kinases; Life Sciences; Na+/H+-exchanging ATPase; Original Article; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Potassium; Proteins; Salinity; Salinity effects; Salinity tolerance; Salt; Salt tolerance; Sesuvium portulacastrum; Sodium; Yeast; Yeasts; Salt tolerance; CBL-interacting protein kinase; Transgenic yeast; Calcineurin B-like protein
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-023-03518-7

Growth and development of plants are greatly affected by salinity. The salt overly sensitive (SOS) pathway plays a vital role in plants responding to salt stress, and has been reported to contain three components: SOS3 (calcineurin B-like protein 4, CBL4), SOS2 (CBL-interacting protein kinase 24, CIPK24) and SOS1. Our previous study demonstrated that transgenic yeast and Arabidopsis could tolerate salt better when expressed with the cell membrane Na + /H + antiporter SOS1 from the halophyte Sesuvium portulacastrum . Here, a new CIPK gene ( SpCIPK8 ) and CBL gene ( SpCBL10 ) have been isolated from S. portulacastrum . The expression of SpCIPK8 and SpCBL10 was induced by salinity in roots of S. portulacastrum . An interaction between SpCBL10 and SpCIPK8 was demonstrated in yeast two-hybrid assays. Subsequent analysis found that SpCBL10 could bind the C-terminus of SpCIPK8. Yeast co-expressing SpSOS1 , SpCIPK8 and SpCBL10 genes grew better and accumulated more potassium (K + ) and less sodium (Na + ) under salt stress than yeast that expressed only one or two of these genes, indicating that Na + was excluded from the cells. Furthermore, we found that the SpCBL10/SpCIPK8 complex regulates the cell membrane Na + /H + antiporter SpSOS1 to enhance yeast salt tolerance by binding the two serine residues at amino acid positions 1144 and 1146 in the conserved DSPS motif at the C-terminus of SpSOS1. Future studies of the SOS pathway will be greatly aided by these results, which suggest some candidate genes for improving plant salt tolerance.