Heterologous expression of Sesuvium portulacastrum SOS-related genes confer salt tolerance in yeast
Growth and development of plants are greatly affected by salinity. The salt overly sensitive (SOS) pathway plays a vital role in plants responding to salt stress, and has been reported to contain three components: SOS3 (calcineurin B-like protein 4, CBL4), SOS2 (CBL-interacting protein kinase 24, CI...
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Published in | Acta physiologiae plantarum Vol. 45; no. 4 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.04.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Growth and development of plants are greatly affected by salinity. The salt overly sensitive (SOS) pathway plays a vital role in plants responding to salt stress, and has been reported to contain three components: SOS3 (calcineurin B-like protein 4, CBL4), SOS2 (CBL-interacting protein kinase 24, CIPK24) and SOS1. Our previous study demonstrated that transgenic yeast and
Arabidopsis
could tolerate salt better when expressed with the cell membrane Na
+
/H
+
antiporter SOS1 from the halophyte
Sesuvium portulacastrum
. Here, a new CIPK gene (
SpCIPK8
) and CBL gene (
SpCBL10
) have been isolated from
S. portulacastrum
. The expression of
SpCIPK8
and
SpCBL10
was induced by salinity in roots of
S. portulacastrum
. An interaction between SpCBL10 and SpCIPK8 was demonstrated in yeast two-hybrid assays. Subsequent analysis found that SpCBL10 could bind the C-terminus of SpCIPK8. Yeast co-expressing
SpSOS1
,
SpCIPK8
and
SpCBL10
genes grew better and accumulated more potassium (K
+
) and less sodium (Na
+
) under salt stress than yeast that expressed only one or two of these genes, indicating that Na
+
was excluded from the cells. Furthermore, we found that the SpCBL10/SpCIPK8 complex regulates the cell membrane Na
+
/H
+
antiporter SpSOS1 to enhance yeast salt tolerance by binding the two serine residues at amino acid positions 1144 and 1146 in the conserved DSPS motif at the C-terminus of SpSOS1. Future studies of the SOS pathway will be greatly aided by these results, which suggest some candidate genes for improving plant salt tolerance. |
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ISSN: | 0137-5881 1861-1664 |
DOI: | 10.1007/s11738-023-03518-7 |