Assessment of Genetic Diversity and Population Structure of Tunisian Barley Accessions (Hordeum vulgare L.) Using SSR Markers

In barley breeding programs, information about genetic dissimilarity and population structure is very important for genetic diversity conservation and new cultivar development. This study aimed to evaluate the genetic variation in Tunisian barley accessions (Hordeum vulgare L.) based on simple seque...

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Bibliographic Details
Published inActa Agrobotanica Vol. 73; no. 4
Main Authors Marzougui, Salem, Kharrat, Mohamed, Mongi ben Younes
Format Journal Article
LanguageEnglish
Published Warsaw Polish Botanical Society 01.01.2020
Subjects
Alleles
barley
Cluster analysis
Cultivars
Genetic analysis
Genetic diversity
Genetic resources
Hordeum vulgare
molecular screening
phylogenetic analysis
Plant breeding
Population genetics
Population structure
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Summary:In barley breeding programs, information about genetic dissimilarity and population structure is very important for genetic diversity conservation and new cultivar development. This study aimed to evaluate the genetic variation in Tunisian barley accessions (Hordeum vulgare L.) based on simple sequence repeat (SSR). A total of 89 alleles were detected at 26 SSR loci. The allele number per locus ranged from two to five, with an average of 3.4 alleles per locus detected from 32 barley accessions, and the average value of polymorphic information content was 0.45. A cluster analysis based on genetic similarity was performed, and the 32 barley resources were classified into five groups. Principal coordinates (PCoA) explained 12.5% and 9.3% of the total variation, and the PCoA was largely consistent with the results of cluster separation of STRUCTURE software analysis. The analysis of genetic diversity in barley collection will facilitate cultivar development and effective use of genetic resources.
ISSN:0065-0951
2300-357X
DOI:10.5586/aa.7343