Inhibitory Mechanisms of Plant Volatile 1-Octanol on the Germination of Aspergillus Flavus Spores
Biogenic volatile organic compounds have promising applications in controlling fungal spoilage of postharvest agro-products and perishable foods. In a previous study, we discovered that the plant volatile 1-octanol showed considerable potential for controlling Aspergillus flavus growth. In this stud...
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Published in | Food biophysics Vol. 19; no. 1; pp. 96 - 108 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.03.2024
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Biogenic volatile organic compounds have promising applications in controlling fungal spoilage of postharvest agro-products and perishable foods. In a previous study, we discovered that the plant volatile 1-octanol showed considerable potential for controlling
Aspergillus flavus
growth. In this study, the inhibitory effects of 1-octanol on the germination of
A. flavus
spores were investigated.
A. flavus
spores did not germinate when exposed to 1.5 µL/mL 1-octanol, and 3.5 µL/mL 1-octanol caused spore death. Biochemical analysis showed that 1-octanol caused a decrease in ergosterol and ATP content, and an increase in hydrogen peroxide and superoxide anion content in a dose-dependent manner. Transcriptomic analysis demonstrated that there were 4117 differentially-expressed genes in
A. flavus
spores exposed to 1.5 µL/mL 1-octanol, mainly enriched in metabolic pathways, steroid biosynthesis, secondary metabolite biosynthesis, ribosomes, glutathione metabolism, the mitogen-activated protein kinases signaling pathway, and pyruvate metabolism. Flow cytometry results showed that 1-octanol treatment resulted in hyperpolarization of the mitochondrial membrane potential, accumulation of reactive oxygen species, and apoptosis. TdT-mediated dUTP nick end labeling/4′,6-diamidino-2-phenylindole double staining and monodansylcadaverine staining results indicated that 1-octanol treatment resulted in DNA fragmentation and induced autophagy, respectively. These results provide new insights into the inhibitory mechanism of 1-octanol on
A. flavus
spore gemination and would facilitate the application of 1-octanol for the protection of postharvest agricultural products. |
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ISSN: | 1557-1858 1557-1866 |
DOI: | 10.1007/s11483-023-09807-5 |