Measurement methods, influencing factors and applications of intercellular receptor-ligand binding kinetics in diseases

• Published in: Progress in biophysics and molecular biology Vol. 194; pp. 43 - 54
• Main Authors: Wu, Qian, Li, Liangchao, Zhang, Yuyan, Ming, Xiaozhi, Feng, Nianjie
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2024
• Subjects: Animals; Application; Disease; Humans; Inflammation - metabolism; Inflammation - pathology; Kinetics; Ligands; Measurement methods; Membrane-protein interactions; Neoplasms - metabolism; Neoplasms - pathology; Protein Binding; Receptor-ligand binding kinetics; Receptors, Cell Surface - metabolism; Measurement methods; Receptor-ligand binding kinetics; Membrane-protein interactions; Application
• ISSN: 0079-6107; 1873-1732; 1873-1732
• DOI: 10.1016/j.pbiomolbio.2024.11.001

Receptor-ligand binding on contacting cells dictates the extent of transmembrane signaling through membrane receptors during cell communication, influencing both the physiological and pathological activities of cells. This process is integral to fundamental biological mechanisms including signal transduction, cancer metastasis, immune responses, and inflammatory cascades, all of which are profoundly influenced by the cell microenvironment. This article provides an overview of the kinetic theory of receptor-ligand binding and examines methods for measuring this interaction, along with their respective advantages and disadvantages. Furthermore, it comprehensively explores the factors that impact receptor-ligand binding, encompassing protein-membrane interactions, the bioelectric microenvironment, auxiliary factors, hydrogen bond strength, pH levels, cis and trans interactions between ligands and receptors. The application of receptor-ligand binding kinetics in various diseases such as immunity, cancer, and inflammation are also discussed. Additionally, the investigation into how functional substances alter receptor-ligand binding dynamics within specific cellular microenvironments presents a promising new approach to treating related diseases. [Display omitted] •Different methods for measuring receptor-ligand binding and their advantages and disadvantages.•Influence of the cellular microenvironment on receptor-ligand binding.•The application of receptor-ligand binding kinetics in immunity, cancer and inflammation.•Strategies for treating diseases by targeting receptor and ligands.