Live cell refractometry using microfluidic devices

Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results...

Full description

Saved in:
Bibliographic Details
Published inOptics letters Vol. 31; no. 18; p. 2759
Main Authors Lue, Niyom, Popescu, Gabriel, Ikeda, Takahiro, Dasari, Ramachandra R, Badizadegan, Kamran, Feld, Michael S
Format Journal Article
LanguageEnglish
Published United States 15.09.2006
Subjects
Cell Shape
HeLa Cells
Humans
Microfluidic Analytical Techniques
Microscopy, Phase-Contrast
Refractometry - instrumentation
Online AccessGet more information

Cover

More Information
Summary:Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
ISSN:0146-9592
DOI:10.1364/OL.31.002759