Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples

We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock ci...

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Bibliographic Details
Published inOptics letters Vol. 31; no. 12; p. 1830
Main Authors Li, Xinhong, Yamauchi, Toyohiko, Iwai, Hidenao, Yamashita, Yutaka, Zhang, Haijun, Hiruma, Teruo
Format Journal Article
LanguageEnglish
Published United States 15.06.2006
Subjects
Equipment Design
Equipment Failure Analysis
Feedback
HeLa Cells
Humans
Image Enhancement - instrumentation
Image Enhancement - methods
Image Interpretation, Computer-Assisted - methods
Interferometry - instrumentation
Interferometry - methods
Light
Microscopy, Phase-Contrast - instrumentation
Microscopy, Phase-Contrast - methods
Reproducibility of Results
Sensitivity and Specificity
Transducers
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Summary:We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock circuit is employed in the system to reduce phase noise. An application to human epithelial cells (HeLa cells) is achieved in our experiment. The advancement of this technique rests in its ability to take images of unstained biological samples quantitatively and on a nanometer scale.
ISSN:0146-9592
DOI:10.1364/OL.31.001830