Luteolibacter yonseiensis sp. nov., isolated from activated sludge using algal metabolites

• Published in: International journal of systematic and evolutionary microbiology Vol. 63; no. Pt 5; pp. 1891 - 1895
• Main Authors: Park, Joonhong, Baek, Gyu Seok, Woo, Sung-Geun, Lee, Jangho, Yang, Jihoon, Lee, Juyoun
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.05.2013
• Subjects: activated sludge; amino acids; Ankistrodesmus; Bacterial Typing Techniques; Base Composition; Cell Wall - chemistry; chemotaxonomy; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - analysis; menaquinones; metabolites; microalgae; microbiology; Molecular Sequence Data; muramic acid; Muramic Acids - analysis; Nucleic Acid Hybridization; nucleotide sequences; Peptidoglycan - analysis; peptidoglycans; Phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Sewage - microbiology; Verrucomicrobia - classification; Verrucomicrobia - genetics; Verrucomicrobia - isolation & purification; Verrucomicrobiaceae; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - analysis
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.046664-0

A Gram-negative, rod-shaped, aerobic bacterial strain, designated EBTL01T, was isolated from activated sludge by using metabolites of microalgae Ankistrodesmus gracilis SAG278-2. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EBTL01T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is related most closely to Luteolibacter pohnpeiensis A4T-83T (95.5 % sequence similarity) and Luteolibacter algae A5J-41-2T (95.2 %). The G+C content of the genomic DNA of strain EBTL01T was 56.3 mol% and the menaquinone MK-9 was detected as the predominant quinone. Major fatty acid components were iso-C14 : 0, C16 : 1ω7c and C16 : 0. The amino acids of the cell-wall peptidoglycan contained muramic acid and meso-diaminopimelic acid. These profile results supported the affiliation of strain EBTL01T to the genus Luteolibacter. On the other hand, based on chemotaxonomic properties and phenotypic characteristics, strain EBTL01T could be clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL01T represents a novel species of the genus Luteolibacter, for which the name Luteolibacter yonseiensis sp. nov. is proposed. The type strain is EBTL01T ( = KCTC 23678T = JCM 18052T).