Response surface methodology for enzymatic hydrolysis optimization of jabon alkaline pulp with Tween 80 surfactant addition

• Published in: Biomass conversion and biorefinery Vol. 12; no. 6; pp. 2165 - 2174
• Main Authors: Nababan, Martua Yan Steward, Fatriasari, Widya, Wistara, Nyoman J
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2022; Springer Nature B.V
• Subjects: Biofuels; Biotechnology; Design optimization; Drying ovens; Energy; Enzymes; Ethanol; Fermentation; Hydrolysis; Original Article; Renewable and Green Energy; Response surface methodology; Saccharification; Surfactants; Wood pulp; Tween 80 surfactant; Bioethanol; Response surface methodology; Jabon wood; Enzymatic hydrolysis
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-020-00807-w

This study was intended to determine the optimum condition for enzymatic hydrolysis of the jabon wood pulp. Ethanol production with separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes were also compared. Jabon was delignified with 25% (w/v) NaOH and the pulp was beaten to a freeness of 600, 400 and 200 mL CSF. Tween 80 surfactant was used to improve the enzymatic hydrolysis. The effect of pulp freeness, enzyme loading, and surfactant concentration on enzymatic hydrolysis was optimized by response surface method according to the Box-Behnken design. Under optimized condition (550 mL CSF pulp freeness, 28 FPU/g enzyme loading and 1.66% Tween 80), the yield of reducing sugar per oven-dry weight (g) of biomass was eight times higher than that of the untreated ones. The ethanol yields improved up to 24%. Separate hydrolysis and fermentation process resulted in a higher ethanol yield. The surfactant addition increases the conversion of cellulose into bioethanol by 27%.