Investigation of the morphological and cytogenetic variations of an intraspecific Asiatic lily hybrid using 5S and 18S rDNA probes

• Published in: Horticulture, environment and biotechnology Vol. 61; no. 2; pp. 339 - 346
• Main Authors: Islam, Md. Mazharul, Yesmin, Reshma, Jung, Min-Jung, Kim, Hong-Yul, Kim, Chang-Kil, Lim, Ki-Byung
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.04.2020; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Color; Cytogenetics; DNA probes; Flowering; Leaves; Life Sciences; Loci; Morphology; Offspring; Parents & parenting; Plant Breeding/Biotechnology; Plant Ecology; Plant Physiology; Pollen; Progeny; Research Report; Ribosomal DNA; Variation; Viability; progeny; FISH; Loci; F; Morphology
• ISSN: 2211-3452; 2211-3460
• DOI: 10.1007/s13580-019-00216-7

This study aimed to determine the morphological and cytogenetic differences in an intraspecific Asiatic F 1 Lilium hybrid. The results indicated that leaf color and shape, flower morphology including color, spot size on petal, and filament color in the F 1 hybrid showed significant variations as compared to parents, while days to flowering were similar to those of the parents. Moreover, stem height, leaf number, and leaf width showed distinct variations. Interestingly, the intraspecific F 1 progeny obtained from Asiatic lily parents showed higher pollen viability. In addition, FISH results revealed significant variation in the number of 18S rDNA and 5S rDNA loci identified in both parents and the F 1 progeny. The female (2n = 4x = 48) parent had 12 loci of 18S rDNA, whereas the male (2n = 2x = 24) parent and the F 1 (2n = 3x = 36) had 8 and 11 loci of 18S rDNA, respectively. Moreover, the F 1 progeny had 9 loci of 5S rDNA compared with the 7 and 4 loci identified in the female and male parents, respectively. All 5S rDNA signals were observed on the long arm in both the female parent and the F 1 ; however, one 5S rDNA signal was observed on the short arm in the male parent. Therefore, this study demonstrated that the distribution of ribosomal DNA was greatly different in the F 1 progeny than that of parents.