A simple “turn-on” fluorescent sensor for reversible recognition of aluminum ion in living cell
A simple and reliable “turn-on” fluorescent sensor (E)-1-[((2-hydroxyethyl)imino) methyl] naphthalen-2-ol ( HNP ) has been designed, synthesized, and characterized by 1 H-NMR, 13 C-NMR, FT-IR, and EI-MS analysis. The binding property of HNP was examined employing UV–Vis and fluorescence spectroscopy...
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Published in | Analytical sciences Vol. 38; no. 9; pp. 1163 - 1169 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Singapore
Springer Nature Singapore
01.09.2022
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Subjects | |
Online Access | Get full text |
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Summary: | A simple and reliable “turn-on” fluorescent sensor (E)-1-[((2-hydroxyethyl)imino) methyl] naphthalen-2-ol (
HNP
) has been designed, synthesized, and characterized by
1
H-NMR,
13
C-NMR, FT-IR, and EI-MS analysis. The binding property of
HNP
was examined employing UV–Vis and fluorescence spectroscopy.
HNP
exhibited high selectivity towards Al
3+
among other cations and anions. The fluorescence titration experiment has established binding stoichiometry of
HNP
with Al
3+
is 2:1, which can be further verified by HR-MS. The detection limit of
HNP
is 2.9 μM, and it can be reversible five-to-seven times to detect Al
3+
without losing much efficiency which indicates that it can be a reliable probe for Al
3+
. Additionally,
HNP
was successfully applied for the detection of Al
3+
in living cells.
Graphical abstract
To achieve the detection of aluminum ion across a simple, reliable, and precise method, we have investigated the reversible detection (which can reversible response to Al
3+
for five-to-seven times) of Al
3+
through an extremely simple (requires only one-step reaction) “turn-on” fluorescent probe which enables us to visualize and analyze Al
3+
with low detection limit (2.9 μM) and high selectivity in living cell without interference from the high abundant small biological molecules. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0910-6340 1348-2246 |
DOI: | 10.1007/s44211-022-00154-y |