Laccase-like nanozymes fabricated by copper and tannic acid for removing malachite green from aqueous solution

• Published in: Colloid and polymer science Vol. 299; no. 10; pp. 1533 - 1542
• Main Authors: Ge, Zhiqiang, Wu, Biping, Sun, Tingting, Qiao, Bin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2021; Springer Nature B.V
• Subjects: Acids; Aqueous solutions; Catalytic activity; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Copper; Design factors; Design of experiments; Dyes; Enzymes; Food Science; Fourier transforms; Hybrid composites; Laccase; Ligands; Malachite green; Nanocomposites; Nanotechnology and Microengineering; Original Contribution; Oxidation; Physical Chemistry; Polymer Sciences; Radiation; Scanning electron microscopy; Soft and Granular Matter; Spectrum analysis; Substrates; Tannic acid; Wastewater treatment; X ray photoelectron spectroscopy; Tannic acid; Nanozyme; Laccases; Copper ions; Malachite green
• ISSN: 0303-402X; 1435-1536
• DOI: 10.1007/s00396-021-04867-w

Seeking simple, green, and inexpensive preparation methods and materials of enzyme mimics has always been highly desirable. In this paper, a nanocomposite with laccase activity was successfully fabricated by using a simple self-association method with copper and tannic acid. Characterizations through SEM, XRD, FTIR, and XPS showed that the Cu-TA hybrid composites had good spherical in shape with a diameter of about 20 nm and three different valence Cu ions (Cu 2+ , Cu + , and Cu 0 ). The catalytic activity was displayed against typical oxidation substrate of laccases, 2,4-DP. Compared to natural laccases, Cu-TA composites were able to better tolerate changes of pH, temperature, ionic strength, and storage conditions. The Cu-TA composites could remove malachite green (MG) from aqueous solutions effectively. The removal efficiency of MG was optimized using a series of batch tests with single-factor experiment design. Under the optimum conditions, the removal efficiency of MG still retained about 90% after 3 times cycles. Therefore, the Cu-TA composites, as a laccases-like nanozyme, have excellent developing potential in wastewater treatment due to the low-cost of TA and simplicity of preparation.