CRISPR-based electrochemical biosensors for animal health: Recent advances

• Published in: Progress in biophysics and molecular biology Vol. 193; pp. 7 - 18
• Main Authors: Gattani, Anil, Mandal, Sanju, Agrawal, Aditya, Patel, Pragati, Jain, Anand Kumar, Singh, Purnima, Garg, Akshay, Mishra, Aditya
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2024
• Subjects: Animal; Biosensor; CRISPR-Cas; Electrochemical; Health; Voltammetry; CRISPR-Cas; Voltammetry; Health; Animal; Biosensor; Electrochemical
• ISSN: 0079-6107; 1873-1732; 1873-1732
• DOI: 10.1016/j.pbiomolbio.2024.09.001

Animal diseases are a major concern to animal welfare, human health and the global economy. Early detection, prevention and control of these animal diseases are crucial to ensure sustainability of livestock sector, to reduce farm losses and protecting public health. Points of care (POC) devices are small, portable instruments that provide rapid results thus reduce the risk of disease transmission and enable early intervention. CRISPR based diagnostics offer more accurate and efficient solution for monitoring animal health due to their quick response, can detect very low level of pathogenic organism or disease markers and specificity. These diagnostics are particularly useful in the in area with limited resources or access to common diagnostic methods, especially in developing countries. The ability of electrochemical sensors to detect accurately very low analyte concentration makes them suitable for POC diagnostics and field application. CRISPR base electrochemical biosensors show great potential in revolutionizing disease detection and diagnosis including animal health. However, challenges, such as achieving selectivity and sensitivity, need to be addressed to enhance the competitiveness of these biosensors. Currently, most CRISPR based bioassay research focuses on nucleic acid target detection, but researchers exploring to monitor small organic/inorganic non-nucleic acid molecules like toxins and proteins. Emerging diagnostics would be centered on CRISPR-Cas system will offer great potential as an accurate, specific and effective means to identify microorganism, virus, toxins, small molecules, peptides and nucleic acid related to various animal health disorders particularly when integrated into electrochemical biosensing platform. •Overviewed the potential of electrochemical CRISPR based diagnostics for monitoring animal health.•Application of cis-clevage activity of CRISPR- Cas system for Electrochemical bioassay has reviewed.•Emphasized the application of Electrochemical CRISPR-Cas system in clinical diagnosis and food safety.