Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool

A new panel of cytogenetically characterized pig-rodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 ind...

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Bibliographic Details
Published inMammalian genome Vol. 7; no. 4; p. 280
Main Authors Zijlstra, C, Bosma, A A, de Haan, N A, Mellink, C
Format Journal Article
LanguageEnglish
Published United States 01.04.1996
Subjects
Animals
Chromosome Mapping - methods
Cricetinae
Cytogenetics
Genetic Linkage
Hybrid Cells
Mice
Microsatellite Repeats
Polymerase Chain Reaction
Swine - genetics
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Summary:A new panel of cytogenetically characterized pig-rodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 independent pig-hamster and 6 independent pig-mouse cell lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2-14, 2-15, 3-9, 14-15, 14-16, and 16-17. The microsatellites tested were S0022, S0023, S0084, S0098, S0112, S0113, S0114, S0115, S0117, S0118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and correlation. Eight microsatellites could be assigned to specific pig chromosomes, confirming seven assignments based on linkage analysis.
ISSN:0938-8990
DOI:10.1007/s003359900083