Effect of cytokinins and sucrose concentration on the efficiency of micropropagation of ‘Zes006’ Actinidia chinensis var. chinensis, a red-fleshed kiwifruit cultivar

• Published in: Plant cell, tissue and organ culture Vol. 138; no. 1; pp. 1 - 10
• Main Authors: Saeiahagh, H., Mousavi, M., Wiedow, Claudia, Bassett, H. B., Pathirana, R.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2019; Springer Nature B.V
• Subjects: Acclimation; Acclimatization; Actinidia; Actinidia chinensis; Adenine; Benzyladenine; Biomedical and Life Sciences; Cultivars; Cytokinins; Genotypes; Kiwifruit; Leaf area; Life Sciences; Media; Micropropagation; Original Article; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Primers; Rooting; Roots; Somaclonal variation; Sucrose; Sugar; Tissue culture; Zeatin; SSR markers; Meta-Topolin; glucosylation; In vitro culture; Somaclonal variation; Shoot proliferation; Acclimation
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-019-01597-4

The effect of N 6 (3-hydroxybenzyl)adenine ( meta -Topolin— m T) was compared with that of N 6 -benzylaminopurine (BAP) and zeatin at the proliferation stage of micropropagation of red-fleshed Actinidia chinensis var. chinensis ‘Zes006’ in two separate experiments. Shoot number, shoot weight, leaf number and leaf area were significantly higher in m T-supplemented media compared with BAP or zeatin. When transferred to rooting media, plantlets that were propagated in m T-supplemented media readily produced roots, enabling easy acclimation to the greenhouse, whereas none of the plantlets propagated in BAP- or zeatin-supplemented media produced roots. Using 12 pairs of Simple Sequence Repeat primers designed for A. chinensis var. chinensis , a very low rate of somaclonal variation was detected at some loci in plantlets produced in zeatin- (1.04%), BAP- (0.4%) as well as in m T- (0.2%) supplemented media. Overall, m T in equimolar concentrations was the better cytokinin for tissue culture of ‘Zes006’ kiwifruit and may well be applicable to many other kiwifruit genotypes. Key message Supplementation of media with meta-Topolin for in vitro propagation of the red-fleshed kiwifruit cultivar ‘Zes006’ enhanced better shoot proliferation, giving healthy plantlets that were easier to acclimatize to the greenhouse environment compared with media supplemented with 6-benzylaminopurine or zeatin. It also induced a lower rate of somaclonal variation, as detected by SSR markers.